Study

PDAC organoid genomic heterogeneity

Study ID Alternative Stable ID Type
EGAS00001006782 Other

Study Description

The establishment of patient-derived pancreatic cancer organoid culture in recent years creates an exciting opportunity for researchers to perform a wide range of in vitro studies on a model that closely recapitulates the tumor. Among the outstanding questions in pancreatic cancer biology are the causes and consequences of genomic heterogeneity observed in the disease. However, to use pancreatic cancer organoids as a model to study genomic variations, we need to first understand the degree of genomic heterogeneity and its stability within organoids. Here, we used single-cell whole-genome sequencing to investigate the genomic heterogeneity of two independent pancreatic cancer organoids, as well as their genomic stability with extended culture. Clonal populations with similar copy number profiles were observed within the organoids, and the proportion of these clones was shifted with extended culture, suggesting the growth advantage of some clones. However, sub-clonal genomic heterogeneity was also observed within each clonal population, indicating the genomic instability of the ... (Show More)

Study Datasets 2 datasets.

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Dataset ID Description Technology Samples
EGAD00001009741
Two primary tumor-derived PDAC organoids were subjected to SNP array, RNA-seq, and single-cell WGS.
Illumina NovaSeq 6000 4
EGAD00010002408
Two primary tumor-derived PDAC organoids were subjected to SNP array, RNA-seq, and single-cell WGS
Illumina Infinium Global Screening Array-24 2

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