ChIP-seq profiling of H3K4me1 and H3K27ac in iPSCs and iPSC-derived endoderm cells in CPRF patients with LSD1 mutations and healthy controls

LSD1 patient (children) and control (healthy fathers) derived iPSCs and endoderm differentiated cells were expanded and harvested for ChIP-seq experiment following the procedure described in Trovato et al, PMID: 39214979. Cells were cross-linked in PFA, sonicated and soluble fractions were collected for IP with H3K4me1 or H3K27ac antibodies. Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Preparation Kit (New England Biolabs) and sequenced on Illumina NextSeq 500 (75 bp in single-end mode).

• Type: Epigenetics
• Archiver: European Genome-phenome Archive (EGA)