SARS-CoV-2 induced alterations of the upper airway DNA methylome exert long-term impacts on ciliary genes involved in ciliary function

Enzymatic DNA methylome (n=33) and single-cell RNA (n=24) sequencing were performed in nasal cells of COVID-19 patients and controls. In addition, we re-sampled a subset of patients for transcriptome analyses at 3 and 12 months post-infection and followed the expression of differentially regulated genes over time.

Type: Epigenetics
Archiver: European Genome-Phenome Archive (EGA)

1 Dataset 1 Publication

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Dataset ID	Description	Technology	Samples
EGAD50000000398	Nasal swabs were collected from COVID-19 patients during the acute phase of infection as well as 3 and 12-months post-infection. Cells isolated from nasal swabs were subjected to whole-genome enzymatic DNA methylation sequencing (n=33) and scRNA-seq (n=24). For n=12 former COVID-19 pateints, follow-up samples were collected at 3 and 12 months post-infection and subjected to scRNA-seq.	Illumina NovaSeq 6000	45

Publications	Citations
DNA methylation changes during acute COVID-19 are associated with long-term transcriptional dysregulation in patients' airway epithelial cells. Messingschlager M, Mackowiak SD, Voelker MT, Bieg M, Loske J, Chua RL, Liebig J, Lukassen S, Thürmann L, Seegebarth A, Twardziok S, Doncevic D, Herrmann C, Lorenz S, Klages S, Steinbeis F, Witzenrath M, Kurth F, Conrad C, Sander LE, Ishaque N, Eils R, Lehmann I, Laudi S, Trump S. EMBO Mol Med 17: 2025 923-937	0

Publications

Citations

DNA methylation changes during acute COVID-19 are associated with long-term transcriptional dysregulation in patients' airway epithelial cells.
Messingschlager M, Mackowiak SD, Voelker MT, Bieg M, Loske J, Chua RL, Liebig J, Lukassen S, Thürmann L, Seegebarth A, Twardziok S, Doncevic D, Herrmann C, Lorenz S, Klages S, Steinbeis F, Witzenrath M, Kurth F, Conrad C, Sander LE, Ishaque N, Eils R, Lehmann I, Laudi S, Trump S. EMBO Mol Med 17: 2025 923-937