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PCNSL single-cell dataset

This dataset includes 4 samples with scRNA-seq and 3 sample with CITE-seq, scTCR-seq and scBCR-seq.

Type: Transcriptome Analysis
Archiver: European Genome-Phenome Archive (EGA)

1 Dataset 1 Publication

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD50000000685	Mononuclear cells were isolated as cell suspensions by dissociation in a BD Horizon™ Dri Tumor & Tissue Dissociation Reagent (BD Biosciences) using a gentleMACS dissociator (Miltenyi Biotec). A totalseq-C antibody cocktail (Biolegend) was used, and the cell suspensions were diluted to a concentration of 1,000 cells/μL. Library sequencing was performed using the DNBSEQ-G400 (MGI Tech), Illumina HiSeq 2000, or Illumina NovaSeq 6000 5 (Illumina). The FASTQ files were aligned to the 10x provided reference genome using 10x Genomics Cell Ranger software v7.1.0 to create unique molecular identifier count tables of gene expression of the samples.	DNBSEQ-G400 Illumina HiSeq 2000 Illumina NovaSeq 6000	7

Publications	Citations
Single-cell and spatial characterization of plasmablast-like lymphoma cells in primary central nervous system lymphoma. Kobayashi H, Chijimatsu R, Naoi Y, Otani Y, Mizuta R, Fujii K, Ishida J, Murakami H, Ujiie H, Ikeuchi K, Urata T, Seike K, Fujiwara H, Asada N, Fujii N, Matsuoka KI, Sato Y, Maeda Y, Ennishi D. Blood Neoplasia 2: 2025 100058	1