The carbonyl nucleobase adduct M3Ade is a potent antigen for adaptive polyclonal MR1-restricted T cells

"Background: M3Ade is a cell endogenous carbonyl adduct of adenine that binds MR1, supports MR1-mediated stimulation of multiple non-MAIT MR1-reactive T cells (MR1T) in vitro and allows ex-vivo identification of MR1Ts via the usage of an MR1-tetramer loaded with M3Ade (MR1-M3Ade tetramer). Goals: to characterize MR1-M3Ade tetramer positive cells by performing single-cell RNA sequencing (scRNA-seq) and single-cell T Cell Receptor sequencing (scTCR-seq) of MR1-M3Ade tetramer positive T cells from PBMCs of 6 healthy donors and of MR1-M3Ade tetramer negative T cells from PBMCs of 5 of the same 6 donors. Details: This EGA study contains the raw data sequencing files for both the scRNA-seq and the scTCR-seq samples."

• Type: Transcriptome Analysis
• Archiver: European Genome-Phenome Archive (EGA)