Changes in CRISPR/Cas9 Outcomes depending on the usage of Pifithrin-alpha

Study of the effects of Pifithrin-alpha in the context of genetic-engineering in primary human T cells.

1 Dataset

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Dataset ID	Description	Technology	Samples
EGAD50000000929	To explore T cell-intrinsic driving forces of CRISPR-induced aneuploidy at the TRAC gene locus, we performed scKaryo-seq (Bolhaqueiro et al., Nature Genetics 2019). We compared the impact of the small molecule pifithrin-alpha in activated TRAC KO T cells. In a separate experiment, we determined the effect of pifithrin-alpha on non-activated T cells edited at the TRAC locus or treated with non-targeting Cas9 RNPs.	NextSeq 2000	14