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Changes in CRISPR/Cas9 Outcomes depending on the usage of Pifithrin-alpha

Study of the effects of Pifithrin-alpha in the context of genetic-engineering in primary human T cells.

Type: Whole Genome Sequencing
Archiver: European Genome-Phenome Archive (EGA)

1 Dataset 1 Publication

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD50000000929	To explore T cell-intrinsic driving forces of CRISPR-induced aneuploidy at the TRAC gene locus, we performed scKaryo-seq (Bolhaqueiro et al., Nature Genetics 2019). We compared the impact of the small molecule pifithrin-alpha in activated TRAC KO T cells. In a separate experiment, we determined the effect of pifithrin-alpha on non-activated T cells edited at the TRAC locus or treated with non-targeting Cas9 RNPs.	NextSeq 2000	14

Publications	Citations
Modulation of TCR stimulation and pifithrin-α improve the genomic safety profile of CRISPR-engineered human T cells. Ursch LT, Müschen JS, Ritter J, Klermund J, Bernard BE, Kolb S, Warmuth L, Andrieux G, Miller G, Jiménez-Muñoz M, Theis FJ, Boerries M, Busch DH, Cathomen T, Schumann K. Cell Rep Med 5: 2024 101846	0