TIRE-seq: an Integrated Sample Extraction and Transcriptomics Workflow for High Throughput Perturbation Studies

TIRE-seq is a novel RNA sequencing method that integrates mRNA purification directly into library preparation, eliminating separate RNA extraction. The technique demonstrated utility across three biological applications, including capturing transcriptional changes in human T cells, identifying genes driving murine dendritic cell differentiation, and analyzing the dose-response effects of temozolomide on patient-derived neurospheres. With its simplified approach and high sequencing efficiency, TIRE-seq offers a cost-effective solution for large-scale gene expression studies.

Type: RNASeq
Archiver: European Genome-Phenome Archive (EGA)

1 Dataset 1 Publication

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD50000001261	TIRE-seq is a novel RNA sequencing method that integrates mRNA purification directly into library preparation, eliminating separate RNA extraction. The technique demonstrated utility across three biological applications, including capturing transcriptional changes in human T cells, identifying genes driving murine dendritic cell differentiation, and analyzing the dose-response effects of temozolomide on patient-derived neurospheres. With its simplified approach and high sequencing efficiency, TIRE-seq offers a cost-effective solution for large-scale gene expression studies.	NextSeq 2000	1

Publications	Citations
TIRE-seq simplifies transcriptomics via integrated RNA capture and library preparation. O'Keeffe P, Nouri Y, Saw HS, Moore Z, Baldwin TM, Olechnowicz SWZ, Jabbari JS, Squire DM, Leslie S, Wang C, You Y, Ritchie ME, Cross RS, Jenkins MR, Audiger C, Naik SH, Whittle JR, Freytag S, Best SA, Hickey PF, Amann-Zalcenstein D, Bowden R, Brown DV. Sci Rep 15: 2025 15385	1