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SATB1 KO Treg and Teff cells: ATAC-seq

To study the global effects of SATB1 on gene networks in fully differentiated, ex vivo expanded human CD4 T effector cells and Treg cells, we performed ATAC-seq. We systematically assessed the impact of SATB1 on the chromatin levels in mature human Treg and CD4 T effector cells.

Type: Gene Regulation Study
Archive: European Genome-phenome Archive (EGA)

1 Dataset 1 Publication

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD50000001276	We expanded human Treg and CD4+ Teff cells for 9 days, re-activated the cells and performed CRISPR/Cas9 RNP-mediated KO at day 11. The cells were treated with a pro-inflammatory cytokine IL-12 on day 12. 4 days after editing, living cells were FACS-sorted, the cell nuclei were extracted and prepared for ATAC-seq. AAVS1 KO served as control. The transcription factors SATB1 and two other targets (included to increase the robustness of the dataset) were knocked-out. Three biological replicates have been applied.	Illumina NovaSeq 6000	24

Publications	Citations
SATB1 is a targetable modulator of JAK-STAT signaling and cytokines in human Treg and Tconv cells. Kolb S, Diekmann L, Lochert EE, Warmuth L, Ritter J, Schmidtke G, Weber M, Hoffmann M, List M, Kotlarz D, Serr I, Daniel C, Busch DH, Schmidl C, Schumann K. EMBO Rep 27: 2026 4029-4053	0