Shared disease specific B cell clones in Crohn’s disease

We performed deep sequencing of the B cell receptor (BCR) repertoire in four cohorts of patients with Crohn’s disease (CD) together with healthy and disease controls. Using sequence homology, we identified BCR clones that were shared between patients with CD but not found in healthy individuals nor in patients with ulcerative colitis (UC), indicating CD-associated B cell immune responses. Shared clones were present in inflamed gut mucosa, draining intestinal lymph nodes and blood, suggesting the presence of common CD-associated antigens that drive B cell responses in CD patients.

Type: Transcriptome Sequencing
Archiver: European Genome-Phenome Archive (EGA)

1 Dataset 1 Publication

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD50000001335	This dataset contains transcriptome data generated from naive and memory B cells isolated from patients with inflammatory bowel disease and healthy controls. Data was generated from both patients with Crohn's disease and ulcerative colitis. In addition, it contains BCR repertoire data from patients with IBD and healthy controls. The repertoire data was generated using amplicon sequencing of RNA isolated from peripheral blood, lymph nodes from inflamed gut and gut associated lymphatic tissue.	Illumina HiSeq 4000 Illumina MiSeq	895

Publications	Citations
Disease-specific B cell clones are shared between patients with Crohn's disease. Kotagiri P, Rae WM, Bergamaschi L, Pombal D, Lee JY, Noor NM, Sojwal RS, Rubin SJS, Unger LW, Tolmeijer SH, Manferrari G, Bashford-Rogers RJM, Bingham DB, Stift A, Rogalla S, Gubatan J, Lee JC, Smith KGC, McKinney EF, Boyd SD, Lyons PA. Nat Commun 16: 2025 3689	3