PBAT sequencing of naïve human ESCs

This project investigates the DNA methylation landscape of naïve human embryonic stem cells (hESCs) derived from human embryos, assessing the impact of ERK inhibition (ERKi). Naïve ESCs were cultured in UXGL or PXGL media. Post bisulphite adaptor tagging protocol was used to profile genome-wide DNA methylation.

Type: Epigenetics
Archiver: European Genome-Phenome Archive (EGA)

1 Dataset 1 Publication

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD50000001475	To assess the DNA methylation landscape of naïve human embryonic stem cells derived from ERKi or control blastocysts using PXGL or UXGL media.	unspecified	16

Publications	Citations
Suppression of ERK signalling promotes pluripotent epiblast in the human blastocyst. Simon CS, McCarthy A, Woods L, Staneva D, Proks M, Salehin N, Lea G, Huang Q, Linneberg-Agerholm M, Faulkner A, Papathanasiou A, Elder K, Snell P, Christie L, Garcia P, Shaikly V, Taranissi M, Choudhary M, Herbert M, Hanna CW, Brickman JM, Niakan KK. Nat Commun 16: 2025 6922	3