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Evaluation of an ex vivo immunotherapy platform using EV-primed immune cells and patient-derived glioblastoma spheroids

This study presents an ex vivo co-culture system integrating glioblastoma stem-like cells (GSCs) and extracellular vesicle (EV)-primed peripheral immune cells to explore immunotherapeutic responses. EVs were derived from mock- or virus-infected GSCs to modulate immune activity. scRNA-seq was used to assess immune cell activation, functional states, and tumor–immune interactions. The platform enables high-resolution analysis of immune dynamics and EV-mediated immunomodulation in glioblastoma.

Type: RNASeq
Archive: European Genome-phenome Archive (EGA)

1 Dataset

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Dataset ID	Description	Technology	Samples
EGAD50000001617	We established an integrated ex vivo preclinical model using 3D organoids composed of patient-derived GSCs co-cultured with peripheral blood mononuclear cells (PBMCs) from healthy donors. PBMCs from healthy donors were pre-treated with either EVOV or control EVs before being introduced into the GSC organoid co-cultures. Single-cell RNA sequencing (scRNA-seq) was performed using ScaleBio’s combinatorial indexing platform, generating libraries sequenced on the NovaSeq 6000 and profiling approximately 60,000 cells across six samples. Data processing included dimensionality reduction, clustering, and annotation of 16 cell subpopulations (5 GSC subtypes and 11 immune cell subsets) using canonical gene markers and unsupervised algorithms. Differential gene expression (DGE) analysis was performed to compare the treatment groups within each cluster. Functional and pathway enrichment analyses were conducted using gene module scoring, dot plot visualization, and enrichment of Gene Ontology and hallmark pathways.	Illumina NovaSeq 6000	6