Multi-modal spatial characterization of tumor-immune microenvironments in diffuse large B-cell lymphoma

We have leveraged spatial transcriptomics together with proteomic and genomic analysis to investigate the spatial immunology and pathobiology of DLBCL. We define distinct cellular niches that differ in frequency across tumors, related to both Epstein-Barr virus (EBV) infection status and the anatomical site of the tumor, and show that residence within different cellular niches is associated with divergent functional states of T-cells and tumor B-cells due to different patterns of cell-cell communication. This provides a framework for understanding stereotyped patterns of spatial organization in DLBCL and immunological interactions associated with heterogeneity in immune cell infiltration and function across patients.

Type: Cancer Genomics
Archiver: European Genome-Phenome Archive (EGA)

1 Dataset 1 Publication

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD50000001641	This dataset includes NGS data of large B-cell lymphoma, including bulk RNAseq data of 71 biopsies and germline WES data of 70 biopsies.	Illumina NovaSeq 6000	141

Publications	Citations
Multi-modal spatial characterization of tumor immune microenvironments identifies targetable inflammatory niches in diffuse large B cell lymphoma. Dai Y, Kizhakeyil A, Chihara D, Li X, Liu Y, Sainz Zuniga TP, Wilson A, Henderson J, Vibe D, Petrosyants A, Jacobson C, Sarachakov A, Nomie K, Kryukov K, Bagaev A, Chauhan A, Westin JR, Flowers CR, Vega F, Wang L, Green MR. Nat Genet 57: 2025 2715-2727	1