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Transcriptome analysis in human testis during ageing

The molecular mechanism allowing the human testis to age without major loss of function remains elusive. In this study, we aimed to investigate the transcriptomic changes taking place in human testis during the ageing process. In order to isolate the effects of ageing, we selected samples with full spermatogenesis and no significant differences in cellular composition. We performed whole transcriptome sequencing and evaluated differential gene expression as well as alternative transcript usage between young (24-31 years old), middle-aged (41-45 years old), and aged (54-75 years old) samples.

Type: Transcriptome Analysis
Archive: European Genome-phenome Archive (EGA)

1 Dataset

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Dataset ID	Description	Technology	Samples
EGAD50000001677	This dataset consists of trancriptome sequencing of human testicular samples with full spermatogenesis but different ages. We evaluated three age groups, namely young (24-31 years old, n=4), middle-aged (41-45 years old, n=3), and aged (54-75 years old, n=6). RNA was isolated from snap-frozen bipsies using Direct-Zol RNA microprep kit and the quality was evaluated with RNA ScreenTape. Samples were rRNA depleted (NEBNext rRNA depletion kit), libraries were prepared (NEBNext Ultra RNA II directional Library Prep kit), and sequenced (2x150 cycles, NextSeq 500). Sequencing files were uploaded as fastq. Sequencing data for samples G1.3 and G1.4 were also used in a previous study (Siebert-Kuss et al., 2022) and are deposited in EGA under accession number EGAS00001006135 as CTR-1 and CTR-3, respectively.	NextSeq 500	11