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Raw fastq files (not trimmed) from RNAseq sequencing

Samples from infants diagnosed with KMT2A-r B-ALL confirmed by comprehensive immunophenotypic and molecular/cytogenetic features. Mononuclear cells containing >87% blasts were isolated by density gradient centrifugation. Immunophenotyping was performed using the monoclonal antibodies CD45-FITC, CD19-APC, and NG2-PE. NG2POS (NG2 positive) and NG2NEG (NG2 Negative) B-ALL blasts (CD45dimCD19+) were sorted by ﬂuorescence-activated cell sorting (FACS) using a FACS-Aria Fusion cell sorter (BD Biosciences).

Type: RNASeq
Archive: European Genome-phenome Archive (EGA)

1 Dataset

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Dataset ID	Description	Technology	Samples
EGAD50000001769	NG2POS and NG2NEG blast populations from 5 KMT2A-r B-ALL samples were isolated by ﬂuorescence-activated cell sorting and RNA was extracted with Maxwell® RNA Isolation kit (Promega, Madison, Wisconsin, USA). The RNASeq libraries were prepared from total RNA using the TruSeq™ RNA Sample Prep Kit v2 (Illumina), and were sequenced on HiSeq2500 platform (Illumina) in paired-end mode	Illumina HiSeq 2500	10