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Single-cell genotype-to-phenotype (scG2P) data of single nuclei from cell line mixing experiment and six donors

We developed Single-cell genotype-to-phenotype (scG2P) to profile mutations in driver gene hotspots and capture mRNA targets of interest, validating this assay in a cell line mixing experiment of KYSE-270, KYSE-410, and HCT-116, and applied the assay to esophageal biopsy samples from six donors.

Type: Transcriptome Sequencing
Archiver: European Genome-phenome Archive (EGA)

1 Dataset 1 Publication

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD50000002066	Single-cell genotype-to-phenotype (scG2P) of normal esophageal epithelium, based on targeted DNA genotyping of mutation hot spots and targeted RNA capture.	Illumina NovaSeq 6000	6

Publications	Citations
Genotype-to-phenotype mapping of somatic clonal mosaicism via single-cell co-capture of DNA mutations and mRNA transcripts. Yuan DJ, Zinno J, Botella T, Dhingra D, Wang S, Hawkins AG, Swett A, Sotelo J, Raviram R, Hughes C, Potenski C, Godfrey KD, Ainsworth KM, Xu S, Que J, Abrams JA, Yokoyama A, Kakiuchi N, Ogawa S, Landau DA. Cancer Discov None: 2025 None	0