Copied to clipboard!

Iso-Seq enables discovery of novel isoform variants in human retina at single cell resolution

We utilized Iso-Seq with full-length sequencing to profile the human retina at single cell resolution for isoform discovery. We generated a retina transcriptome dataset consisting of 25,302 nuclei from three donor retina, and identified 73,166 known isoforms and 27,880 novel isoforms across 10 major cell types.

Type: Transcriptome Sequencing
Archive: European Genome-phenome Archive (EGA)

1 Dataset

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD50000002227	This dataset contains single-nucleus RNA sequencing data from human retinal tissue generated using long-read sequencing approach. Single nuclei from three donor retinas were captured using the 10x Genomics Chromium system to generate barcoded cDNA libraries. PacBio Iso-Seq data derived from re-amplified full-length cDNA enabled transcript isoform reconstruction. The dataset includes transcriptomic profiles of 25,302 nuclei across 10 major retinal cell types, with comprehensive detection of known and novel isoforms, providing a high-resolution resource for isoform-level analyses in the human retina.	Sequel II	3