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μSeq: Universal mutation rate quantification via deep sequencing of a single clonal expansion

We use patient-derived colorectal cancer organoids to introduce µSeq, a universal framework for inferring mutation rates in diverse biological systems. Our approach extracts mutation rates from deep sequencing of single clonal expansions, with a time gain of tenfold or more compared to a mutation-accumulation line, at the cost of three billion read whole-genome sequencing.

Type: Whole Genome Sequencing
Archiver: European Genome-phenome Archive (EGA)

1 Dataset

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Dataset ID	Description	Technology	Samples
EGAD50000002528	High-depth WGS was performed on two clonal organoids, one ancestor and one endpoint of a single lineage in a mutation accumulation experiment, and of the same clonal organoid that has been engrafted and grown in xenografts during the accumulation period. Paired sequencing was performed on a Novaseq X Plus with 150bp reads aiming at 500Gbp - 150x. 6 fastq files have been deposited for the 3 samples	Illumina NovaSeq X	3