Single-molecule nanopore sequencing of plasma and cerebrospinal fluid cell-free DNA from paediatric patients with brain and extracranial solid tumours.

Cell-free DNA (cfDNA) profiling enables minimally invasive cancer detection and monitoring. Here, we report single-molecule nanopore sequencing data generated using the SIMMA assay for 792 plasma and cerebrospinal fluid cfDNA samples from 277 paediatric patients with diverse brain and extracranial tumours. SIMMA is a low-input single-molecule sequencing approach that enables multimodal whole-genome and high-depth targeted sequencing of the same cfDNA sample for both tumour-agnostic and tumour-informed liquid biopsy analysis (Sauer et al. 2026).

• Type: Whole Genome Sequencing
• Archive: European Genome-phenome Archive (EGA)