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Mutant TP53 allelic states in human hematopoietic stem and progenitor cells

Clonal hematopoiesis driven by TP53 mutations is a potent risk factor for diverse human pathologies. By CRISPR/Cas9 engineering of human hematopoietic stem and progenitor cells, we investigate the role of TP53 mutations in induction of chromosomal aberrations by single-cell template strand sequencing.

Type: Whole Genome Sequencing
Archive: European Genome-phenome Archive (EGA)

1 Dataset 1 Publication

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Dataset ID	Description	Technology	Samples
EGAD50000002846	Clonal hematopoiesis driven by TP53 mutations is a potent risk factor for diverse human pathologies. By CRISPR/Cas9 engineering of human hematopoietic stem and progenitor cells, we investigate the role of TP53 mutations in induction of chromosomal aberrations by single-cell template strand sequencing. Single-cell template strand sequencing of a collection of cord blood samples gene edited to carry mono- or bi-allelic TP53 mutations, to study development of chromosome instability. The experiment comprises 3 donors and 6 genotypes: R175H/WT, R175H/KO, R273H/WT, R273H/KO, KO/WT and KO/KO. AAVS1 safe harbor targeting was used as control. For each donor+genotype combination, samples were split in two treatment conditions: 10nM doxorubicin or DMSO control.	NextSeq 500	507

Publications	Citations
Mutant TP53 allelic states in human hematopoietic stem and progenitor cells. Lind K, Dutta S, Cosenza MR, Eder T, Foßelteder J, Pabst G, Schöll W, Prokesch A, Auner HW, Wölfler A, Zebisch A, Reinisch A, Grebien F, Korbel JO, Sill H. Leukemia 40: 2026 2602-2606	0