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Mutant TP53 allelic states in human hematopoietic stem and progenitor cells

Clonal hematopoiesis driven by TP53 mutations is a potent risk factor for diverse human pathologies. By CRISPR/Cas9 engineering of human hematopoietic stem and progenitor cells, we investigate the role of TP53 mutations in induction of chromosomal aberrations by single-cell template strand sequencing.

Type: Whole Genome Sequencing
Archive: European Genome-phenome Archive (EGA)

1 Dataset

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Dataset ID	Description	Technology	Samples
EGAD50000002846	Clonal hematopoiesis driven by TP53 mutations is a potent risk factor for diverse human pathologies. By CRISPR/Cas9 engineering of human hematopoietic stem and progenitor cells, we investigate the role of TP53 mutations in induction of chromosomal aberrations by single-cell template strand sequencing. Single-cell template strand sequencing of a collection of cord blood samples gene edited to carry mono- or bi-allelic TP53 mutations, to study development of chromosome instability. The experiment comprises 3 donors and 6 genotypes: R175H/WT, R175H/KO, R273H/WT, R273H/KO, KO/WT and KO/KO. AAVS1 safe harbor targeting was used as control. For each donor+genotype combination, samples were split in two treatment conditions: 10nM doxorubicin or DMSO control.	NextSeq 500	507