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Single-cell transcriptomic profiling of ASPM-mutant and isogenic control human cortical organoids

This study investigates the impact of pathogenic ASPM disruption on human cortical development using an isogenic human iPSC-derived cortical organoid model. Single-cell RNA sequencing was performed on control CtAH4 organoids and the CRISPR/Cas9-engineered ASPM-mutant derivative M5AH4-3a at differentiation days 36, 56 and 70. Libraries were generated using 10X Genomics Chromium Single Cell 3′ v3.1 Next GEM chemistry and sequenced on MGISEQ-2000 and Illumina NovaSeq 6000 platforms.

Type: RNASeq
Archive: European Genome-phenome Archive (EGA)

1 Dataset

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Dataset ID	Description	Technology	Samples
EGAD50000003154	Raw paired-end single-cell RNA-sequencing data from control AH4 and isogenic ASPM-mutant human cortical organoids collected at differentiation days 36, 56 and 70. Libraries were generated using 10X Genomics Chromium Single Cell 3′ v3.1 Next GEM chemistry and sequenced on MGISEQ-2000 and Illumina NovaSeq 6000 platforms.	Illumina NovaSeq 6000 MGISEQ-2000RS	6