Gene Fusion Discovery through RNA Sequencing of Human Glioblastoma Stem Cell Lines
We used massively parallel, paired-end sequencing of expressed transcripts (RNA-seq) to detect novel gene fusions in short-term cultures of glioma stem-like cells freshly isolated from nine patients carrying primary glioblastoma multiforme (GBM). The culture of primary GBM tumors under serum-free conditions selects cells that retain phenotypes and genotypes closely mirroring primary tumor profiles as compared to serum-cultured glioma cell lines that have largely lost their developmental identities.
- Type: RNA Sequencing
- Archiver: The database of Genotypes and Phenotypes (dbGaP)