Molecular Identity of Human Outer Radial Glia Cells During Cortical Development

Study ID Alternative Stable ID Type
phs000989 Cohort

Study Description

We sought to characterize cellular heterogeneity in the human cortical germinal zone at a molecular level during peak stages of cortical neurogenesis. We captured single cells without enrichment and generated sequencing libraries using the C1TM Single-Cell Auto Prep System (Fluidigm), the SMARTer Ultra Low RNA Kit (Clontech), and the Nextera XT DNA Sample Preparation Kit (Illumina). We performed unbiased clustering of the single cells and further examined transcriptional variation among cell groups interpreted as radial glia. Within this population, the major sources of variation related to cell cycle progression and the stem cell niche from which radial glia were captured. We found that outer subventricular zone radial glia (oRG cells) preferentially express genes related to extracellular matrix formation, migration, and stemness, including TNC, PTPRZ1, FAM107A, HOPX, and LIFR and related this transcriptional state to the position, morphology, and cell behaviors previously used to classify the cell type. Our results suggest that oRG cells ... (Show More)

Archive Link Archive Accession
dbGaP phs000989

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