High-throughput determination of the antigen specificities of T cell receptors in single cells

Study ID Alternative Stable ID Type
phs001678 Single Cell Analysis

Study Description

High-throughput linking of T cell receptor (TCR) sequences to their binding antigens is vital for immune profiling, yet challenging. We present Tetramer associated TCR Sequencing (TetTCR-Seq) to address this challenge. Binding is determined using a library of DNA-barcoded antigen tetramers that are rapidly and inexpensively generated using an in vitro transcription/translation platform. We included CMV+ donors (CMV seropositive donors who are infected with Cytomegalovirus) to screen for CMV specific TCRs.

Archive Link Archive Accession
dbGaP phs001678

Who archives the data?

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