Modulation of Lung Immune Responses to Viral Infection-Microbiome Interactions with Respiratory Organoids

Here, we colonized bronchial epithelial air-liquid interface tissue cultures with 58 different individual microbes. Following 18 and 48 hours of colonization, we performed bulk RNA-seq of the host epithelial cells (n=3). Overall, we found significant microbial species-level and strain-level variation in host gene expression changes for innate immunity and epithelial barrier genes. Of particular interest, we found significant heterogeneity in stimulation of host interferon and antiviral interferon-stimulated genes.

Study registered in ImmPort under accession # SDY2281.

• Type: Control Set
• Archiver: The database of Genotypes and Phenotypes (dbGaP)