RNA Sequencing of Surgically Resected Colorectal Liver Metastases

Sean Pitroda
spitroda1@bsd.uchicago.edu

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Dataset ID	Description	Technology	Samples
EGAD00001004111	Reverse-stranded paired-end 75 base-pair RNA sequencing libraries of 93 metastatic FFPE samples were constructed using Illumina Total RNA Stranded Kits. Ribosomal RNAs (rRNAs) were depleted by using the Ribo-Zero rRNA Removal Kit (Illumina). Libraries were sequenced on a HiSEQ2500 machine. Five samples were re-sequenced using paired-end 50 base-pair libraries due to the smaller insert sizes.	Illumina HiSeq 2500	93