Developmental & Stem Cell Biology

Dac ID Contact Person Email Access Information
EGAC00001001002 Michael D. Taylor mdtaylor [at] sickkids [dot] ca No additional information is available

This DAC controls 3 datasets:

Dataset ID Description Technology Samples
EGAD00001004318 We used single-cell transcriptomics to study >60,000 cells from the developing murine cerebellum, and show that different molecular subgroups of childhood cerebellar tumors mirror the transcription of cells from distinct, temporally restricted cerebellar lineages. Illumina HiSeq 2000,Illumina HiSeq 2500,Illumina HiSeq 4000 47
EGAD00001004347 We analyzed alternative splicing with Shh medulloblastoma. This dataset contains bam files of whole genome sequencing from 4 cases. Genomic DNA was isolated from both tumor and matched control specimens. We performed whole genome sequence on Illumina Hiseq. Illumina HiSeq 2000 8
EGAD00001006305 To further understand the biology of Sonic hedgehog medulloblastoma and its molecular subtypes, we studied 250 human Shh-MB using strand-specific RNA sequencing. We identified novel alterations within the cAMP dependent pathway and found that 18% of tumors have genetic events that directly target the abundance and/or stability of MYCN. We also discovered an extensive network of fusions in focally amplified regions, and several loss-of-function fusions in tumor suppressor genes PTCH, SUFU and NCOR1. Molecular convergence on a core of specific genes by nucleotide variants, copy number aberrations, and gene fusions highlights key roles of specific pathways in the pathogenesis of Sonic hedgehog medulloblastoma. Illumina HiSeq 2000,Illumina HiSeq 2500 82