DAC
Developmental & Stem Cell Biology
Dac ID | Contact Person | Access Information | |
---|---|---|---|
EGAC00001001002 | Michael D. Taylor | mdtaylor [at] sickkids [dot] ca | No additional information is available |
This DAC controls 3 datasets:
Dataset ID | Description | Technology | Samples |
---|---|---|---|
EGAD00001004318 | We used single-cell transcriptomics to study >60,000 cells from the developing murine cerebellum, and show that different molecular subgroups of childhood cerebellar tumors mirror the transcription of cells from distinct, temporally restricted cerebellar lineages. | Illumina HiSeq 2000,Illumina HiSeq 2500,Illumina HiSeq 4000 | 47 |
EGAD00001004347 | We analyzed alternative splicing with Shh medulloblastoma. This dataset contains bam files of whole genome sequencing from 4 cases. Genomic DNA was isolated from both tumor and matched control specimens. We performed whole genome sequence on Illumina Hiseq. | Illumina HiSeq 2000 | 8 |
EGAD00001006305 | To further understand the biology of Sonic hedgehog medulloblastoma and its molecular subtypes, we studied 250 human Shh-MB using strand-specific RNA sequencing. We identified novel alterations within the cAMP dependent pathway and found that 18% of tumors have genetic events that directly target the abundance and/or stability of MYCN. We also discovered an extensive network of fusions in focally amplified regions, and several loss-of-function fusions in tumor suppressor genes PTCH, SUFU and NCOR1. Molecular convergence on a core of specific genes by nucleotide variants, copy number aberrations, and gene fusions highlights key roles of specific pathways in the pathogenesis of Sonic hedgehog medulloblastoma. | Illumina HiSeq 2000,Illumina HiSeq 2500 | 82 |