DAC

The University of Hong Kong Colon Cancer Organoids Genomics Study Data Access Committee

Dac ID Contact Person Email Access Information
EGAC00001001405 Suet Yi Leung suetyi [at] hku [dot] hk No additional information is available

This DAC controls 3 datasets:

Dataset ID Description Technology Samples
EGAD00001005753 Four micrograms of total RNA was used for cDNA library construction using the KAPA Stranded mRNA-Seq Kit (KR0960-v3.15), following manufacturer's protocol. The adaptor-ligated libraries were enriched by 6 cycles of polymerase chain reaction (PCR). Libraries were sequenced using the Novaseq 6000 with paired end 151bp reads. Illumina HiSeq 1500,Illumina NovaSeq 6000 55
EGAD00001005754 Five hundred fifty nanograms of genomic DNA were input for library preparation after fragmentation by Covaris S2, following the KAPA Hyper Prep Kit (KR0961-V1.14) protocols, with selection for a library size range of 250-450 bp. Three hundred nanograms per library DNA each from 12 samples were normalized and combined into a single pool for exome capture using the xGen Lockdown Probes and Reagents based on their standard protocols HiSeq X Ten,Illumina HiSeq 1500,Illumina NovaSeq 6000 117
EGAD00001005759 Five hundred nanograms of genomic DNA was fragmented by Covaris S2, the fragmented DNAs were performed end-repair, A-tailing at the 3 prime end, adaptors ligation with an IDT dual-indexed UMI adaptor system at the terminal ends. The adapter ligated library with size range 300-750bp were selected by dual-SPRI method. Twenty percent of the size selected PCR-free libraries were enriched by 5 PCR cycles prior to library size assessment by Bioanalyzer Fragment Analyzer. The PCR-free libraries were quantified by qPCR.The PCR-free libraries were denatured and diluted to optimal concentration. Illumina NovaSeq 6000 was used for Pair-End 151bp sequencing. Illumina NovaSeq 6000 9