DAC
The University of Hong Kong Colon Cancer Organoids Genomics Study Data Access Committee
Dac ID | Contact Person | Access Information | |
---|---|---|---|
EGAC00001001405 | Suet Yi Leung | suetyi [at] hku [dot] hk | No additional information is available |
This DAC controls 3 datasets:
Dataset ID | Description | Technology | Samples |
---|---|---|---|
EGAD00001005753 | Four micrograms of total RNA was used for cDNA library construction using the KAPA Stranded mRNA-Seq Kit (KR0960-v3.15), following manufacturer's protocol. The adaptor-ligated libraries were enriched by 6 cycles of polymerase chain reaction (PCR). Libraries were sequenced using the Novaseq 6000 with paired end 151bp reads. | Illumina HiSeq 1500,Illumina NovaSeq 6000 | 55 |
EGAD00001005754 | Five hundred fifty nanograms of genomic DNA were input for library preparation after fragmentation by Covaris S2, following the KAPA Hyper Prep Kit (KR0961-V1.14) protocols, with selection for a library size range of 250-450 bp. Three hundred nanograms per library DNA each from 12 samples were normalized and combined into a single pool for exome capture using the xGen Lockdown Probes and Reagents based on their standard protocols | HiSeq X Ten,Illumina HiSeq 1500,Illumina NovaSeq 6000 | 117 |
EGAD00001005759 | Five hundred nanograms of genomic DNA was fragmented by Covaris S2, the fragmented DNAs were performed end-repair, A-tailing at the 3 prime end, adaptors ligation with an IDT dual-indexed UMI adaptor system at the terminal ends. The adapter ligated library with size range 300-750bp were selected by dual-SPRI method. Twenty percent of the size selected PCR-free libraries were enriched by 5 PCR cycles prior to library size assessment by Bioanalyzer Fragment Analyzer. The PCR-free libraries were quantified by qPCR.The PCR-free libraries were denatured and diluted to optimal concentration. Illumina NovaSeq 6000 was used for Pair-End 151bp sequencing. | Illumina NovaSeq 6000 | 9 |