Colon Cancer Organoid Cultures and Tumors Whole Genome Sequencing Data

Dataset ID Technology Samples
EGAD00001005759 Illumina NovaSeq 6000 9

Dataset Description

Five hundred nanograms of genomic DNA was fragmented by Covaris S2, the fragmented DNAs were performed end-repair, A-tailing at the 3 prime end, adaptors ligation with an IDT dual-indexed UMI adaptor system at the terminal ends. The adapter ligated library with size range 300-750bp were selected by dual-SPRI method. Twenty percent of the size selected PCR-free libraries were enriched by 5 PCR cycles prior to library size assessment by Bioanalyzer Fragment Analyzer. The PCR-free libraries were quantified by qPCR.The PCR-free libraries were denatured and diluted to optimal concentration. Illumina NovaSeq 6000 was used for Pair-End 151bp sequencing.

Who controls access to this dataset

For each dataset that requires controlled access, there is a corresponding Data Access Committee (DAC) who determine access permissions. Access to actual data files is not managed by the EGA. If you need to request access to this data set, please contact:

The University of Hong Kong Colon Cancer Organoids Genomics Study Data Access Committee
Contact person: Suet Yi Leung
Email: suetyi [at] hku [dot] hk
More details: EGAC00001001405


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