DAC

Tytgat Institute for Liver and Intestinal Research Data Access Committee

Dac ID Contact Person Email Access Information
EGAC00001001483 Wouter J. de Jonge w [dot] j [dot] dejonge [at] amsterdamumc [dot] nl No additional information is available

This DAC controls 1 dataset:

Dataset ID Description Technology Samples
EGAD00001005959 In this experiment we investigated the effect of HDAC3 inhibition on the transcriptome of IFNg-primed macrophages under different tolerization conditions. Peripheral blood mononuclear cells (PBMCs) were isolated from 3 healthy donors. PBMCs were isolated from whole blood of healthy donors using Ficoll gradient (Invitrogen). Monocytes (CD14+ cells) were positively selected from PBMCs using CD14 Microbeads according to the manufacturer’s instructions (Miltenyi Biotec). Monocytes were subsequently treated with or without 500 nM HDAC3i (ITF3100) for 30 minutes prior to overnight IFNg priming (50 ng/mL). Cells were then kept without LPS (non-LPS; N), treated with 10 ng/mL LPS once (non-tolerized; NT), or treated with LPS twice (tolerized; T; second LPS concentration: 100 ng/mL). In total, there were 18 samples included. Illumina HiSeq 4000 18