Need Help?

RNA-seq of non-LPS treated (N), non-tolerized (NT), and tolerized (T) IFNg-primed macrophages pretreated with or without HDAC3i

In this experiment we investigated the effect of HDAC3 inhibition on the transcriptome of IFNg-primed macrophages under different tolerization conditions. Peripheral blood mononuclear cells (PBMCs) were isolated from 3 healthy donors. PBMCs were isolated from whole blood of healthy donors using Ficoll gradient (Invitrogen). Monocytes (CD14+ cells) were positively selected from PBMCs using CD14 Microbeads according to the manufacturer’s instructions (Miltenyi Biotec). Monocytes were subsequently treated with or without 500 nM HDAC3i (ITF3100) for 30 minutes prior to overnight IFNg priming (50 ng/mL). Cells were then kept without LPS (non-LPS; N), treated with 10 ng/mL LPS once (non-tolerized; NT), or treated with LPS twice (tolerized; T; second LPS concentration: 100 ng/mL). In total, there were 18 samples included.

Request Access

Controlled access to data generated at the Tytgat Institute for Liver and Intestinal Research, Amsterdam UMC, University of Amsterdam.

- The User Institution agrees to protect the confidentiality of Research Participants in any research papers or publications that they prepare by taking all reasonable care to limit the possibility of identification. - The User Institution agrees not to link or combine these Data to other information or archived data available in a way that could re-identify the Research Participants, even if access to that data has been formally granted to the User Institution or is freely available without restriction. Any user requesting access to the data generated at the Tytgat Institute for Liver and Intestinal Research must apply for authorization, which is granted by the Tytgat Institute for Liver and Intestinal Research Data Access Committee (DAC). Decisions to grant access are made based on whether the request conforms to the specifications within the Data Access Agreement

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001004218 RNASeq

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00003446593 fastq.gz 1.5 GB
EGAF00003446594 fastq.gz 1.6 GB
EGAF00003446595 fastq.gz 1.7 GB
EGAF00003446596 fastq.gz 1.5 GB
EGAF00003446597 fastq.gz 1.6 GB
EGAF00003446598 fastq.gz 1.7 GB
EGAF00003446599 fastq.gz 1.6 GB
EGAF00003446600 fastq.gz 1.5 GB
EGAF00003446601 fastq.gz 1.7 GB
EGAF00003446602 fastq.gz 1.5 GB
EGAF00003446603 fastq.gz 1.3 GB
EGAF00003446604 fastq.gz 1.6 GB
EGAF00003446605 fastq.gz 1.6 GB
EGAF00003446606 fastq.gz 1.4 GB
EGAF00003446607 fastq.gz 1.4 GB
EGAF00003446608 fastq.gz 1.6 GB
EGAF00003446609 fastq.gz 1.7 GB
EGAF00003446610 fastq.gz 1.6 GB
EGAF00003446651 bam 838.0 MB
EGAF00003446652 bai 2.4 MB
EGAF00003446653 bam 985.3 MB
EGAF00003446654 bai 2.4 MB
EGAF00003446655 bam 958.6 MB
EGAF00003446656 bai 2.4 MB
EGAF00003446705 bam 846.1 MB
EGAF00003446706 bai 2.4 MB
EGAF00003446707 bam 1.0 GB
EGAF00003446708 bai 2.4 MB
EGAF00003446709 bam 986.1 MB
EGAF00003446710 bai 2.4 MB
EGAF00003446711 bam 1.1 GB
EGAF00003446712 bai 2.4 MB
EGAF00003446713 bam 966.4 MB
EGAF00003446714 bai 2.5 MB
EGAF00003446755 bam 991.9 MB
EGAF00003446756 bai 4.1 MB
EGAF00003446757 bam 866.5 MB
EGAF00003446758 bai 2.4 MB
EGAF00003446759 bam 999.1 MB
EGAF00003446760 bai 2.5 MB
EGAF00003446761 bam 1.0 GB
EGAF00003446762 bai 2.5 MB
EGAF00003446763 bam 1.0 GB
EGAF00003446764 bai 2.5 MB
EGAF00003446765 bam 1.0 GB
EGAF00003446766 bai 2.5 MB
EGAF00003446876 bam 922.3 MB
EGAF00003446877 bai 2.4 MB
EGAF00003446878 bam 961.0 MB
EGAF00003446879 bai 2.5 MB
EGAF00003446880 bam 997.1 MB
EGAF00003446881 bai 2.5 MB
EGAF00003446882 bam 941.8 MB
EGAF00003446883 bai 2.4 MB
54 Files (45.4 GB)