DAC

Institut National pour la Recherche Biomedicale Comite d'Access aux Donnees

Dac ID Contact Person Email Access Information
EGAC00001001539 Prof Dieudonne Mumba Ngoyi mumbadieudonne [at] yahoo [dot] fr No additional information is available

This DAC controls 2 datasets:

Dataset ID Description Technology Samples
EGAD00010001905 234 samples genotyped at 15 loci LGC Genomics, Hoddesden, UK using the PCR-based KASP assay (Semagn,e tal (2014). Single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP): overview of the technology and its application in crop improvement. Mol Breeding 33, 1–14.) PCR-based KASP assay 233
EGAD00010001906 234 samples genotyped at 40 loci using the MassArrayiPLEX genotyping assay using the iPLEX Gold genotyping kit (Agena Biosciences, cat. 10148-2) Gabriel et al. (2009) [Gabriel, S. , Ziaugra, L. and Tabbaa, D. (2009), SNP Genotyping Using the Sequenom MassARRAY iPLEX Platform. Current Protocols in Human Genetics, 60: 2.12.1-2.12.18. doi:10.1002/0471142905.hg0212s60]). Products were detected on a MassArray mass spectrophotometer and data were acquired in real time with MassArray RT software 4.0.0.2 (Agena Biosciences). SNP clustering and validation was carried out with Typer 4.0.26.75 software (Agena Biosciences). MassArrayiPLEX genotyping assay using the iPLEX Gold genotyping kit 233