Milieu Intérieur Data Access Committee

Darragh Duffy
milieuinterieudac@pasteur.fr

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Dataset ID	Description	Technology	Samples
EGAD00001004979	The dataset reports the 16S rRNA gene sequencing of the fecal microbiota of donors from the Milieu Intérieur Cohort. The Milieu Intérieur cohort includes a total of 1,000 healthy individuals of western European ancestry, recruited in France as part of the Milieu Intérieur project. To assess their fecal microbiota composition, 16S rRNA profiles were generated from stool samples of 863 of the 1,000 donors. Human stool samples were produced at home no more than 24 hours before the scheduled medical visit and collected in a double-lined sealable bag maintaining strict anaerobic conditions. Upon reception at the clinical site, the fresh stool samples were aliquoted and stored immediately at -80°C. DNA was extracted from stool and barcoding PCR was carried out using indexed primers targeting the V3-V5 region of the 16S rRNA gene. Equal volumes of normalized PCR reaction were pooled and thoroughly mixed. The amplicon libraries were sequenced on Illumina MiSeq.	Illumina MiSeq	1311
EGAD00001006554	This dataset includes 1,359 paired-end shotgun metagenomics samples from 946 healthy donors of the Milieu Intérieur cohort. 413 of the donors provided two samples (V1 and V2).	Illumina HiSeq 2500	1359
EGAD00010001489	Genotype data for 5,699,237 genotyped and imputed SNPs in the 816 healthy donors of the Milieu IntÃ©rieur cohort		816