16S-based fecal microbiota composition

Dataset ID Technology Samples
EGAD00001004979 Illumina MiSeq 1311

Dataset Description

The dataset reports the 16S rRNA gene sequencing of the fecal microbiota of donors from the Milieu Intérieur Cohort. The Milieu Intérieur cohort includes a total of 1,000 healthy individuals of western European ancestry, recruited in France as part of the Milieu Intérieur project. To assess their fecal microbiota composition, 16S rRNA profiles were generated from stool samples of 863 of the 1,000 donors. Human stool samples were produced at home no more than 24 hours before the scheduled medical visit and collected in a double-lined sealable bag maintaining strict anaerobic conditions. Upon reception at the clinical site, the fresh stool samples were aliquoted and stored immediately at -80°C. DNA was extracted from stool and barcoding PCR was carried out using indexed primers targeting the V3-V5 region of the 16S rRNA gene. Equal volumes of normalized PCR reaction were pooled and thoroughly mixed. The amplicon libraries were sequenced on Illumina MiSeq.

Data Use Conditions


See further information on Data Use Conditions

Label Code Version Modifier
general research use and clinical care DUO:0000005 2019-01-07
not for profit use only DUO:0000018 2019-01-07
ethics approval required DUO:0000021 2019-01-07
user specific restriction DUO:0000026 2019-01-07
project specific restriction DUO:0000027 2019-01-07
institution specific restriction DUO:0000028 2019-01-07
return to database or resource DUO:0000029 2019-01-07