DAC

PopCol data access commitee

Dac ID Contact Person Email Access Information
EGAC00001002028 Anna Andreasson anna [dot] andreasson [at] ki [dot] se No additional information is available

This DAC controls 1 dataset:

Dataset ID Description Technology Samples
EGAD00001007071 PopCol is a cohort study in Stockholm, Sweden that includes a data-rich set of individuals with data available from bowel symptoms questionnaires, gastroenterology visits and biospecimens (genotype and 16S sequencing from blood and stool samples, respectively). Genotyping was carried out using the Illumina HumanOmniExpressExome-8v1 arrays at the SciLifeLab NGI facility in Uppsala, Sweden. Fecal DNA was extracted from samples kept at -80¬įC using Qiagen 5 QIAamp DNA Stool Mini Kits and analyzed using 16S rRNA gene amplicon sequencing (in the V1-V2 hypervariable region). This was performed on the Illumina MiSeq platform at the Institute of Clinical Molecular Biology (IKMB) in Kiel, Germany. Of these, six PopCol participants were PPI users and 12 used antibiotics. The study was approved by the local Committee of Research Ethics (Forskningskommitt√© Syd) at Karolinska Institutet, Stockholm, in November 2001. Written informed consent was obtained from all participants Illumina MiSeq 134