DAC

CMVB KU Leuven DAC

Dac ID Contact Person Email Access Information
EGAC00001002416 Kathleen Freson kathleen [dot] freson [at] kuleuven [dot] be No additional information is available

This DAC controls 1 dataset:

Dataset ID Description Technology Samples
EGAD00001008356 RNA sequencing data of in vitro differentiated megakaryocyte cells transduced with E527K and WT SRC. CD34+ hematopoietic stem cells (HSC) were isolated from healthy controls before transduction with WT-SRC and E527K-SRC lentiviral vectors in triplicate and differentiation to MK. Three replicates each of two pools were generated for both WT and E527K SRC transduced cells, resulting in 3 WT pool 1 samples, 3 WT pool 2 samples, 3 E527K pool 1 samples and 3 E527K pool 2 samples for a total of 12 samples. RNA was extracted and sequenced with following parameters: Platform: Illumina HiSeq4000, Library Prep Kit: TruSeq stranded mRNA, Sequencing Kit: Illumina HiSeq4000 100 cycles (76-8-8-7), Fragments: single end / fr-firststrand. Illumina HiSeq 4000 12