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RNA sequencing data of in vitro differentiated megakaryocyte cells transduced with E527K and WT SRC

RNA sequencing data of in vitro differentiated megakaryocyte cells transduced with E527K and WT SRC. CD34+ hematopoietic stem cells (HSC) were isolated from healthy controls before transduction with WT-SRC and E527K-SRC lentiviral vectors in triplicate and differentiation to MK. Three replicates each of two pools were generated for both WT and E527K SRC transduced cells, resulting in 3 WT pool 1 samples, 3 WT pool 2 samples, 3 E527K pool 1 samples and 3 E527K pool 2 samples for a total of 12 samples. RNA was extracted and sequenced with following parameters: Platform: Illumina HiSeq4000, Library Prep Kit: TruSeq stranded mRNA, Sequencing Kit: Illumina HiSeq4000 100 cycles (76-8-8-7), Fragments: single end / fr-firststrand.

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DAA terms and conditions for RNA sequencing data

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Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001005808 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00005719883 fastq.gz 1.6 GB
EGAF00005719884 fastq.gz 1.7 GB
EGAF00005719885 fastq.gz 1.5 GB
EGAF00005719886 fastq.gz 1.7 GB
EGAF00005719887 fastq.gz 1.5 GB
EGAF00005719888 fastq.gz 1.7 GB
EGAF00005719889 fastq.gz 1.8 GB
EGAF00005719890 fastq.gz 1.8 GB
EGAF00005719891 fastq.gz 1.7 GB
EGAF00005719892 fastq.gz 1.6 GB
EGAF00005719893 fastq.gz 1.7 GB
EGAF00005719894 fastq.gz 1.6 GB
12 Files (19.9 GB)