DAC

MLL DAC

Dac ID Contact Person Email Access Information
EGAC00001002523 Torsten Haferlach torsten [dot] haferlach [at] mll [dot] com No additional information is available

This DAC controls 1 dataset:

Dataset ID Description Technology Samples
EGAD00001008558 For the cohort of 59 samples, we performed TruSeq DNA PCR-Free whole-genome sequencing library preparation according to manufacturer’s instructions (llumina, ILMN, San Diego, CA) on the automated NGS Star liquid handling platform (Hamilton, Bonaduz, Switzerland) followed by 2x150 bp paired-end sequencing on the HiSeqX or NovaSeq6000 (ILMN). An average coverage of >100x was achieved. For whole transcriptome analysis, the TruSeq Total Stranded RNA kit was used, starting with 250 ng of total RNA, to generate RNA libraries following the manufacturer’s recommendations (ILMN). 2x100bp paired-end reads were sequenced on the NovaSeq 6000 with a median of 50 mio. reads per sample (ILMN). Illumina NovaSeq 6000 59