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MD Anderson Lymphoma & Myeloma Data Access Committee

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Michael Green
mgreen5@mdanderson.org

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This DAC controls 5 datasets

Dataset ID	Description	Technology	Samples
EGAD00001009774	Bone marrow aspirates were obtained from patients with relapsed/refractory large B cell lymphoma (rrLBCL), mononuclear cells isolated by ficoll density-gradient centrifugation, and loaded onto a 10X Chromium for single cell RNA-sequencing using 5’ chemistry without prior cryopreservation. Healthy donor bone marrow mononuclear cells were obtained from healthy allogeneic stem cell transplant donors and analyzed following viable cryopreservation.	Illumina NovaSeq 6000	22
EGAD50000000201	Single cell full transcriptome sequencing of CD19 CAR T-cell infusion products used for standard of care treatment for relapsed/refractory large B-cell lymphoma.	Illumina HiSeq 4000	59
EGAD50000001491	The dataset comprises multi-omics profiles from 232 biopsies, including 217 from patients with large B-cell lymphoma (114 newly diagnosed and 103 relapsed/refractory) and 15 benign control samples. Specifically: single-nucleus RNA sequencing (snRNA-seq) was performed on all 232 biopsies; single-nucleus ATAC sequencing was conducted on 96 biopsies; bulk RNA sequencing was generated for 208 biopsies; whole-exome sequencing (WES) was performed on 174 tumor biopsies with 118 matched germline samples; Low-pass whole-genome sequencing (lpWGS) was performed on 174 tumor biopsies with 41 matched germline samples. All raw data files are provided in FASTQ format.	Illumina NovaSeq 6000	1062
EGAD50000001641	This dataset includes NGS data of large B-cell lymphoma, including bulk RNAseq data of 71 biopsies and germline WES data of 70 biopsies.	Illumina NovaSeq 6000	141
EGAD50000002866	Relapsed/refractory large B-cell lymphoma (rrLBCL) following CAR T-cell therapy remains associated with a poor prognosis. Using single-cell RNA sequencing (scRNA-seq), we characterized the T-cell profiles in 7 post-CAR T rrLBCL and 5 CAR T-naïve rrLBCL, revealing dysfunctional transcriptional features in T cells after CAR T-cell therapy. In addition, the dataset includes T cell profiling of CAR T-cell products and samples obtained after serial killing assays in vitro, performed with or without supplementation with non-IL2Rα agonist pegenzileukin. These analyses demonstrated that IL-2 was the most depleted cytokine following the coculture assay.	Illumina NovaSeq 6000	73