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Francis Crick Institute Data Access Committee

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Dr Douglas T Gray
human-biology@crick.ac.uk

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This DAC controls 4 datasets

Dataset ID	Description	Technology	Samples
EGAD50000001109	Samples were derived from five lines of human iPSC-derived (hiPSC-derived) astrocytes, both alone and in co-culture with neurons +/- alpha-synuclein oligomer treatment. Paired-end FASTQ files for each of the samples are provided.	Illumina NovaSeq 6000	44
EGAD50000001110	Samples were derived from an human iPSC-derived astrocyte line, both alone and in co-culture with neurons +/- alpha-synuclein oligomer treatment.	Illumina HiSeq 4000	6
EGAD50000001361	This dataset contains bulk RNA-seq data from 12 autosomally isogenic human iPSCs. Three biological replicas from XXY (sample ID 419295-7), XX (sample ID 419298-419300), XY (sample ID 419302, 419305-6), and XO (sample ID 419308, 419310, 419312) hiPSC. RNA for this dataset was collected using Trizol method, and polyA library type was implemented. The libraries were sequenced using Illumina device. The sample reads are deposited as fastq files. Analysis have been done using reference alignment to GRCh38 reference genome.	Illumina NovaSeq 6000	12
EGAD50000001362	This dataset contains Whole exome sequence generated using human skin fibroblast sample (ID 419281) from Klinefelter XXY syndrome patient fibroblasts. These fibroblasts were used to generate autosomally isogenic human iPSCs. The sample DNA was collected using commercial kit and Human All Exon v5 capture paired library was sequenced using Illumina HiSeq device. The fastq file (ID 493224) and analysis (ID 3209) bam files have been deposited.	Illumina HiSeq 4000	1