Huch Lab, Max Planck Institute of Molecular Cell Biology and Genetics

Dr Fabian Rost
rost@mpi-cbg.de

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Dataset ID	Description	Technology	Samples
EGAD50000001453	Sample number: 8 Sample details: Total 8 samples, including Homeostatic-like assembloids (4 samples), Fibrotic-like assembloids (4 samples).	Illumina NovaSeq 6000	8
EGAD50000001454	Sample number: 40 Sample details:Total 40 samples, including hepatocyte organoids (74 samples), primary human hepatocytes (15 samples), cholangicocyte organoids (11 samples), hepatocytes cultured in monlayer (5 samples) and portal fibroblasts (3 samples).	Illumina NovaSeq 6000	101
EGAD50000001464	Liver ductal organoids expanded in either conventional (Huch et al., 2015) or refined medium were split and seeded either in the same medium or in DM+. They were sequenced on day 10 after splitting. Droplet-based scRNA-seq was performed using the 10x Genomics Chromium Single Cell Kit v3, and libraries were sequenced on an Illumina NovaSeq 6000 S4. Raw sequencing data were processed using Cell Ranger software (v6.0.1) with the human genome reference (hg38) and gene annotation (Ensembl 98). Dataset integration and downstream analysis were mainly performed using Seurat (v4.3.0.1) with R v4.1.3 “One Push-up”.	Illumina NovaSeq 6000	12