BCR-HGBCL-DH-BCL2 project DAC

Dr Stefano Casola
stefano.casola@ifom.eu

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Dataset ID	Description	Technology	Samples
EGAD50000001523	PCR-based VDJ profiling in IGH+ and IGHUND HGBCL-DH-BCL2(-BCL6) lymphomas	Illumina MiSeq	22
EGAD50000001524	GCB DLBCL MB2 DE and HGBCL-DH-BCL2(-BCL6) cases classified according to IGH status in IGH+ or IGH-undetectable	NextSeq 550	22
EGAD50000001525	High-throughput sequencing of IGHG1 alleles amplified from genomic DNA and the IGG1 (IGVH-CG1) functional transcript, flanking the gRNA-targeted region, from IGHUND HGBCL-DH-BCL2 COH-DHL1 cell line. IGHG1 targeting gRNAs were cloned into lentiCRISPRv2-Puro lentiviral vector (Addgene #98290). COH-DHL1 transduced cells were selected in Puromycin for 7 days, and expanded for further 17 days in the absence of any antibiotic selection.	Illumina MiSeq	6
EGAD50000001526	NGS of 5'RACE IGKV/IGLV PCR-products from IGH+ and IGHUND HGBCL-DH-BCL2(-BCL6) lymphomas	Illumina MiSeq	14
EGAD50000001527	HGBCL-DH-BCL2(-BCL6) lymphoma cases, classified according to IGH status in IGH+ or IGH-undetectable	Illumina NovaSeq 6000	12
EGAD50000001528	FL-HGBCL-DH-BCL2(-BCL6) metachronous lymphoma pairs, classified according to IGH status in IGH+ or IGH-undetectable	Illumina NovaSeq 6000	6