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HighIGHG1 CRISPR-Cas9 edited alleles in IGHUND COH-DHL1 HGBCL2-DH-BCL2 cells

High-throughput sequencing of IGHG1 alleles amplified from genomic DNA and the IGG1 (IGVH-CG1) functional transcript, flanking the gRNA-targeted region, from IGHUND HGBCL-DH-BCL2 COH-DHL1 cell line. IGHG1 targeting gRNAs were cloned into lentiCRISPRv2-Puro lentiviral vector (Addgene #98290). COH-DHL1 transduced cells were selected in Puromycin for 7 days, and expanded for further 17 days in the absence of any antibiotic selection.

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IFOM-ETS controlled-access data

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Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS50000001047 Cancer Genomics

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
i
EGAF50000381729 fastq.gz 3.3 MB
EGAF50000381730 fastq.gz 3.9 MB
EGAF50000381731 fastq.gz 3.3 MB
EGAF50000381732 fastq.gz 3.9 MB
EGAF50000381733 fastq.gz 3.7 MB
EGAF50000381734 fastq.gz 4.2 MB
EGAF50000381735 fastq.gz 2.7 MB
EGAF50000381736 fastq.gz 3.1 MB
EGAF50000381737 fastq.gz 2.9 MB
EGAF50000381738 fastq.gz 3.5 MB
EGAF50000381739 fastq.gz 2.4 MB
EGAF50000381740 fastq.gz 3.0 MB
12 Files (39.9 MB)