DAC: LUMC NeuroD AON off target analysis

Willeke M.C. van Roon-Mom
w.m.c.van_roon@lumc.nl

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Dataset ID	Description	Technology	Samples
EGAD50000001742	Neuronal cells derived from female healthy control hiPSC lines LUMCi003-A, LUMCi003-B and LUMCi023-A were transfected with in total four different splice-switching AONs targeting three different human transcripts. Untransfected samples as well as samples transfected with a scrambled AON were included as controls. Three days after transfection, RNA was isolated for sequencing using Illumina Novaseq6000 in order to perform differential gene expression analysis and differential splicing analysis to determine off-target effects. Sequencing was performed in two batches: one containing the samples from cell line LUMCi003-A and the other containing cell lines LUMCi003-B and LUMCi023-A.	Illumina NovaSeq 6000	61