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As the EGA is co-managed by the EMBL-EBI and CRG, files could be located in the UK and/or Spain, as indicated by their respective flags.

Alternatively, files could be located in one of the international Federated EGA nodes, as indicated next to the node's flag.
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Determining off-target effects of splice-switching antisense oligonucleotides using short read RNAseq in neuronally differentiated human induced pluripotent stem cells

Neuronal cells derived from female healthy control hiPSC lines LUMCi003-A, LUMCi003-B and LUMCi023-A were transfected with in total four different splice-switching AONs targeting three different human transcripts. Untransfected samples as well as samples transfected with a scrambled AON were included as controls. Three days after transfection, RNA was isolated for sequencing using Illumina Novaseq6000 in order to perform differential gene expression analysis and differential splicing analysis to determine off-target effects. Sequencing was performed in two batches: one containing the samples from cell line LUMCi003-A and the other containing cell lines LUMCi003-B and LUMCi023-A.

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AON off target analysis

Controlled access required because the study concerns linked phenotype and genotype data from human subjects which should not be traced back to the donors. Citation plus acknowledgement will be required

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS50000001222 RNASeq

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
i
EGAF00008866176 fastq.gz 4.1 GB
EGAF00008866377 fastq.gz 3.9 GB
EGAF00008866378 fastq.gz 4.4 GB
EGAF00008866379 fastq.gz 4.9 GB
EGAF00008866380 fastq.gz 3.8 GB
EGAF00008866381 fastq.gz 4.8 GB
EGAF00008866382 fastq.gz 5.1 GB
EGAF00008866383 fastq.gz 5.0 GB
EGAF00008866384 fastq.gz 4.6 GB
EGAF00008866385 fastq.gz 4.0 GB
EGAF00008866386 fastq.gz 5.3 GB
EGAF00008866387 fastq.gz 4.2 GB
EGAF00008866388 fastq.gz 3.9 GB
EGAF00008866389 fastq.gz 4.6 GB
EGAF00008866390 fastq.gz 4.1 GB
EGAF00008866391 fastq.gz 5.1 GB
EGAF00008866392 fastq.gz 4.2 GB
EGAF00008866393 fastq.gz 4.4 GB
EGAF00008866394 fastq.gz 6.9 GB
EGAF00008866395 fastq.gz 4.0 GB
EGAF00008866396 fastq.gz 4.1 GB
EGAF00008866397 fastq.gz 3.9 GB
EGAF00008866398 fastq.gz 4.0 GB
EGAF00008866399 fastq.gz 4.9 GB
EGAF00008866400 fastq.gz 4.4 GB
EGAF00008866401 fastq.gz 4.6 GB
EGAF00008866402 fastq.gz 4.9 GB
EGAF00008866403 fastq.gz 4.3 GB
EGAF00008866404 fastq.gz 5.5 GB
EGAF00008866405 fastq.gz 4.4 GB
EGAF00008866406 fastq.gz 3.9 GB
EGAF00008866407 fastq.gz 4.3 GB
EGAF00008866408 fastq.gz 4.4 GB
EGAF00008866409 fastq.gz 6.6 GB
EGAF00008866410 fastq.gz 4.5 GB
EGAF00008866411 fastq.gz 4.1 GB
EGAF00008866412 fastq.gz 4.5 GB
EGAF00008866413 fastq.gz 4.0 GB
EGAF00008866414 fastq.gz 4.0 GB
EGAF00008866415 fastq.gz 5.0 GB
EGAF00008866416 fastq.gz 4.8 GB
EGAF00008866417 fastq.gz 4.3 GB
EGAF00008866418 fastq.gz 4.5 GB
EGAF00008866419 fastq.gz 4.3 GB
EGAF00008866420 fastq.gz 4.7 GB
EGAF00008866421 fastq.gz 3.8 GB
EGAF00008866422 fastq.gz 5.2 GB
EGAF00008866423 fastq.gz 4.7 GB
EGAF00008866424 fastq.gz 4.8 GB
EGAF00008866425 fastq.gz 3.9 GB
EGAF00008866426 fastq.gz 4.0 GB
EGAF00008866427 fastq.gz 4.2 GB
EGAF00008866428 fastq.gz 4.3 GB
EGAF00008866429 fastq.gz 3.6 GB
EGAF00008866430 fastq.gz 7.6 GB
EGAF00008866431 fastq.gz 9.4 GB
EGAF00008866432 fastq.gz 3.8 GB
EGAF00008866433 fastq.gz 3.8 GB
EGAF00008866434 fastq.gz 3.7 GB
EGAF00008866435 fastq.gz 5.0 GB
EGAF00008866436 fastq.gz 7.2 GB
EGAF00008866437 fastq.gz 4.8 GB
EGAF00008866438 fastq.gz 5.0 GB
EGAF00008866439 fastq.gz 5.0 GB
EGAF00008866441 fastq.gz 7.7 GB
EGAF00008866442 fastq.gz 3.9 GB
EGAF00008866443 fastq.gz 7.9 GB
EGAF00008866444 fastq.gz 9.6 GB
EGAF00008866446 fastq.gz 4.7 GB
EGAF00008866448 fastq.gz 5.3 GB
EGAF00008866449 fastq.gz 3.9 GB
EGAF00008866450 fastq.gz 7.5 GB
EGAF00008866451 fastq.gz 8.1 GB
EGAF00008866452 fastq.gz 7.3 GB
EGAF00008866453 fastq.gz 7.3 GB
EGAF00008866454 fastq.gz 7.7 GB
EGAF00008866455 fastq.gz 7.3 GB
EGAF00008866457 fastq.gz 8.3 GB
EGAF00008866458 fastq.gz 4.9 GB
EGAF00008866460 fastq.gz 7.6 GB
EGAF00008866464 fastq.gz 7.4 GB
EGAF00008866465 fastq.gz 7.6 GB
EGAF00008866466 fastq.gz 7.0 GB
EGAF00008866467 fastq.gz 8.1 GB
EGAF00008866468 fastq.gz 7.3 GB
EGAF00008866472 fastq.gz 7.4 GB
EGAF00008866473 fastq.gz 7.9 GB
EGAF00008866475 fastq.gz 8.0 GB
EGAF00008866477 fastq.gz 7.9 GB
EGAF00008866478 fastq.gz 7.8 GB
EGAF00008866479 fastq.gz 7.4 GB
EGAF00008866481 fastq.gz 17.2 GB
EGAF00008866483 fastq.gz 7.4 GB
EGAF00008866484 fastq.gz 7.3 GB
EGAF00008866485 fastq.gz 7.8 GB
EGAF00008866486 fastq.gz 7.6 GB
EGAF00008866487 fastq.gz 17.8 GB
EGAF00008866490 fastq.gz 8.3 GB
EGAF00008866491 fastq.gz 7.0 GB
EGAF00008872091 fastq.gz 8.3 GB
EGAF00008872092 fastq.gz 8.1 GB
EGAF00008872095 fastq.gz 7.7 GB
EGAF00008872096 fastq.gz 7.6 GB
EGAF00008872098 fastq.gz 7.3 GB
EGAF00008872099 fastq.gz 7.5 GB
EGAF00008872101 fastq.gz 9.8 GB
EGAF00008872102 fastq.gz 8.5 GB
EGAF00008872103 fastq.gz 10.2 GB
EGAF00008872104 fastq.gz 8.5 GB
EGAF00008872106 fastq.gz 9.4 GB
EGAF00008872107 fastq.gz 11.8 GB
EGAF00008872109 fastq.gz 10.6 GB
EGAF00008872110 fastq.gz 12.3 GB
EGAF00008872111 fastq.gz 9.2 GB
EGAF00008872112 fastq.gz 7.6 GB
EGAF00008872113 fastq.gz 10.6 GB
EGAF00008872115 fastq.gz 9.3 GB
EGAF00008872116 fastq.gz 8.8 GB
EGAF00008872117 fastq.gz 11.0 GB
EGAF00008872119 fastq.gz 8.9 GB
EGAF00008872120 fastq.gz 9.8 GB
EGAF00008872121 fastq.gz 10.1 GB
122 Files (784.3 GB)