Tracking the stages of human B cell differentiation using single-cell RNA sequencing using an optimized in vitro model

Dr Fabienne Desmots
fabienne.desmots-loyer@univ-rennes.fr

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Dataset ID	Description	Technology	Samples
EGAD50000002118	Tonsils B Cell from three different donors were cultured on our in vitro differentiation model. A mean of 20,000 viable human B cells per donor were hashed with cell-surface oligo (HTO) barcodes and pooled at days 0, 6, 8, and 10 of culture. Single-cell 5’ gene expression libraries and V(D)J enriched libraries were performed using Genomics Chromium Single Cell Immune Profiling Solution (10x Genomics) according to the standard manufacturer’s protocol. Libraries were sequenced on an Illumina NovaSeq 6000 (2 × 150 bp) with an average depth of ≥50,000 reads per cell.	Illumina NovaSeq 6000	4