About file location

As the EGA is co-managed by the EMBL-EBI and CRG, files could be located in the UK and/or Spain, as indicated by their respective flags.

Alternatively, files could be located in one of the international Federated EGA nodes, as indicated next to the node's flag.
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B cell differentiation single cell profiling

Tonsils B Cell from three different donors were cultured on our in vitro differentiation model. A mean of 20,000 viable human B cells per donor were hashed with cell-surface oligo (HTO) barcodes and pooled at days 0, 6, 8, and 10 of culture. Single-cell 5’ gene expression libraries and V(D)J enriched libraries were performed using Genomics Chromium Single Cell Immune Profiling Solution (10x Genomics) according to the standard manufacturer’s protocol. Libraries were sequenced on an Illumina NovaSeq 6000 (2 × 150 bp) with an average depth of ≥50,000 reads per cell.

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DUO:0000042
version: 2021-02-23

general research use

This data use permission indicates that use is allowed for general research use for any research purpose.

Data policy to access ScRNAseq of normal B cell

Data requests must be sent to thierry.fest@univ-rennes.fr fabienne.desmots-loyer@univ-rennes.fr gersende.lacombe@univ-rennes.fr

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS50000001470 RNASeq

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
i
EGAF50000509101 fastq.gz 7.8 GB
EGAF50000509102 fastq.gz 18.1 GB
EGAF50000509103 fastq.gz 12.0 GB
EGAF50000509104 fastq.gz 9.8 GB
EGAF50000509105 fastq.gz 27.9 GB
EGAF50000509106 fastq.gz 23.2 GB
EGAF50000509107 fastq.gz 10.0 GB
EGAF50000509108 fastq.gz 23.4 GB
8 Files (132.3 GB)