EGA_DAC_for_humanFetalMembrane_snRNASeq

Dr Martin Ehrbar
martin.ehrbar@uzh.ch

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Dataset ID	Description	Technology	Samples
EGAD50000002439	This dataset contains raw single-nucleus RNA sequencing (snRNA-seq) data and associated metadata from human fetal membrane samples collected across gestation. Amnion and chorion tissues were obtained from pregnancies spanning midterm to term, including both healthy and preeclampsia cases. Nuclei were isolated from frozen tissue, fixed, and processed using the Parse Biosciences Evercode WT platform for combinatorial barcoding–based library preparation. Libraries were sequenced to generate transcriptome-wide gene expression profiles. Due to the multiplexed experimental design, sequencing data are provided as pooled FASTQ files. Individual biological samples are registered separately, and a mapping file is included to describe the relationship between samples, sequencing runs, and the pooled libraries. Additional metadata files provide sample-level annotations, including tissue type (amnion or chorion), gestational stage, donor identifier, sex, and clinical condition	Illumina NovaSeq X	37