Colossus DAC

Dr Rodrigo Dienstmann
rdienstmann@vhio.net

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Dataset ID	Description	Technology	Samples
EGAD50000003002	Single-nuclei RNA sequencing of human colorectal cancer samples. Nuclei were isolated on ice, followed by single-nuclei RNA-sequencing library preparation using the Chromium Single Cell 3’ reagent kit v3.1 (10x Genomics, Pleasanton, CA) Fastq files, N=18	Illumina NovaSeq 6000	18
EGAD50000003003	Whole exome sequencing of colorectal cancer samples from stage 2/3 human patients. Whole-genome DNA libraries were prepared with the KAPA HyperPlus kit (Roche, Cape Town, South Africa), and exome fragments were captured using the SureSelect Human All Exon V7 kit (Agilent, Hangzhou, China) Fastq files, N=88	Illumina NovaSeq 6000	88
EGAD50000003004	Bulk RNA sequencing of colorectal cancer samples from stage 2/3 human patients. RNA extraction using Qiagen RNeasy FFPE Extraction kit (Qiagen, UK), followed by RNA sequencing library generation with the QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) (Illumina, USA). Fastq files, N=295	Illumina HiSeq 4000	295
EGAD50000003005	Low-coverage whole genome sequencing of colorectal cancer samples from stage 2/3 human patients. Tumor DNA from biopsies was extracted using the QIAamp circulating nucleic acid kit (Qiagen, UK), followed by generation of shot-gun whole genome libraries using the KAPA library preparation kit (KAPA Biosystems). Fastq files, N=295	Illumina HiSeq 4000	295