Use of Deep Sequencing to Dectect Clonal Mutations In Sun Exposed Skin Epidermis PART2

This study aims to define the landscape of somatic mutations in sun exposed human skin by deep sequencing, analyse their frequency and use the data to infer the effect of mutations on proliferating cell behaviour. The frequency of each mutation will reflect the size of the clone of cells in the tissue sample. By analyzing small samples, clones with as few as 100 cells will be detectable. Allele frequency distributions for each mutation will be used to infer cell fate using published methods (Klein et al. 2010). This study will shed unprecedented light on the early clonal events that lead to the emergence of cancer.

• 24/08/2016
• Technology: Illumina HiSeq 2000
• 454 samples
• DAC: EGAC00001000000

• Data Use Conditions:
• IS DUO:0000028 (version: 2021-02-23) institution specific restriction This data use modifier indicates that use is limited to use within an approved institution.
• US DUO:0000026 (version: 2021-02-23) user specific restriction This data use modifier indicates that use is limited to use by approved users.
• GRU DUO:0000042 (version: 2021-02-23) general research use This data use permission indicates that use is allowed for general research use for any research purpose.
• PUB DUO:0000019 (version: 2021-02-23) publication required This data use modifier indicates that requestor agrees to make results of studies using the data available to the larger scientific community.