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SINGLE CELL ANALYSIS OF IN VITRO ERYTHROPOIESIS

We are developing a protocol to differentiate mouse and human induced pluripotent stem (IPS) and embryonic stem (ES) cells towards the haematopoietic pathway to generate erythrocytes in vitro. This system has many applications such as the study of the role of specific genes and human polymorphisms in infectious diseases such as malaria, as well as haematological diseases such as myelodysplastic syndrome. The nature of the in vitro differentiation process means that a heterogeneous population of cells is generated. In order to understand the types of cells produced with our protocol, we have performed a single cell analysis, which has the power to reveal the different populations of cells and their characteristics. For this, a cDNA library has been made that needs to be sequenced to obtain the gene expression profiles of the different cells. With this information we will be able to assess the quality of the differentiation protocol and improve it in order to produce better cells for the downstream applications.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

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Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001000764 Transcriptome Analysis

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00000595280 cram 123.0 MB
EGAF00000595281 cram 123.8 MB
EGAF00000595282 cram 169.1 MB
EGAF00000595283 cram 168.8 MB
EGAF00000595284 cram 155.3 MB
EGAF00000595285 cram 164.0 MB
EGAF00000595286 cram 168.6 MB
EGAF00000595287 cram 161.7 MB
EGAF00000595288 cram 184.1 MB
EGAF00000595289 cram 132.0 MB
EGAF00000595290 cram 148.5 MB
EGAF00000595291 cram 141.9 MB
EGAF00000595292 cram 171.5 MB
EGAF00000595293 cram 183.7 MB
EGAF00000595294 cram 190.2 MB
EGAF00000595295 cram 158.7 MB
EGAF00000595296 cram 203.5 MB
EGAF00000595297 cram 226.6 MB
EGAF00000595298 cram 206.1 MB
EGAF00000595299 cram 42.3 MB
EGAF00000595300 cram 199.9 MB
EGAF00000595301 cram 60.4 MB
EGAF00000595302 cram 158.0 MB
EGAF00000595303 cram 183.0 MB
EGAF00000595304 cram 177.0 MB
EGAF00000595305 cram 266.7 MB
EGAF00000595306 cram 223.4 MB
EGAF00000595307 cram 241.6 MB
EGAF00000595308 cram 250.3 MB
EGAF00000595309 cram 236.5 MB
EGAF00000595310 cram 235.0 MB
EGAF00000595311 cram 270.2 MB
EGAF00000595312 cram 216.1 MB
EGAF00000595313 cram 190.7 MB
EGAF00000595314 cram 178.8 MB
EGAF00000595315 cram 204.5 MB
EGAF00000595316 cram 223.5 MB
EGAF00000595317 cram 213.2 MB
EGAF00000595318 cram 244.8 MB
EGAF00000595319 cram 282.3 MB
EGAF00000595320 cram 329.1 MB
EGAF00000595321 cram 216.4 MB
EGAF00000595322 cram 274.3 MB
EGAF00000595323 cram 281.1 MB
EGAF00000595324 cram 219.8 MB
EGAF00000595325 cram 197.8 MB
EGAF00000595326 cram 232.3 MB
EGAF00000595327 cram 223.4 MB
EGAF00000595328 cram 234.9 MB
EGAF00000595329 cram 366.0 MB
EGAF00000595330 cram 341.6 MB
EGAF00000595331 cram 156.4 MB
EGAF00000595332 cram 227.8 MB
EGAF00000595333 cram 252.0 MB
EGAF00000595334 cram 337.7 MB
EGAF00000595335 cram 190.0 MB
EGAF00000595336 cram 245.7 MB
EGAF00000595337 cram 229.9 MB
EGAF00000595338 cram 219.8 MB
EGAF00000595339 cram 152.9 MB
EGAF00000595340 cram 172.6 MB
EGAF00000595341 cram 249.9 MB
EGAF00000595342 cram 240.7 MB
EGAF00000595343 cram 212.7 MB
EGAF00000595344 cram 259.8 MB
EGAF00000595345 cram 188.0 MB
EGAF00000595346 cram 223.1 MB
EGAF00000595347 cram 258.5 MB
EGAF00000595348 cram 196.2 MB
EGAF00000595349 cram 268.8 MB
EGAF00000595350 cram 202.5 MB
EGAF00000595351 cram 204.8 MB
EGAF00000595352 cram 210.7 MB
EGAF00000595353 cram 341.1 MB
EGAF00000595354 cram 211.6 MB
EGAF00000595355 cram 259.5 MB
EGAF00000595356 cram 171.3 MB
EGAF00000595357 cram 240.0 MB
EGAF00000595358 cram 267.1 MB
EGAF00000595359 cram 271.4 MB
EGAF00000595360 cram 224.1 MB
EGAF00000595361 cram 239.6 MB
EGAF00000595362 cram 251.9 MB
EGAF00000595363 cram 183.3 MB
EGAF00000595364 cram 238.5 MB
EGAF00000595365 cram 225.2 MB
EGAF00000595366 cram 333.7 MB
EGAF00000595367 cram 252.0 MB
EGAF00000595368 cram 193.5 MB
EGAF00000595369 cram 267.2 MB
EGAF00000595370 cram 280.2 MB
EGAF00000595371 cram 228.7 MB
EGAF00000595372 cram 290.6 MB
EGAF00000595373 cram 304.4 MB
EGAF00000595374 cram 171.8 MB
EGAF00000595375 cram 156.1 MB
EGAF00000595376 cram 236.4 MB
EGAF00000595377 cram 169.6 MB
EGAF00000595378 cram 169.6 MB
EGAF00000595379 cram 156.3 MB
EGAF00000595380 cram 164.9 MB
EGAF00000595381 cram 169.7 MB
EGAF00000595382 cram 163.1 MB
EGAF00000595383 cram 185.0 MB
EGAF00000595384 cram 132.8 MB
EGAF00000595385 cram 149.0 MB
EGAF00000595386 cram 142.9 MB
EGAF00000595387 cram 172.6 MB
EGAF00000595388 cram 184.1 MB
EGAF00000595389 cram 190.5 MB
EGAF00000595390 cram 158.7 MB
EGAF00000595391 cram 204.1 MB
EGAF00000595392 cram 227.1 MB
EGAF00000595393 cram 206.5 MB
EGAF00000595394 cram 42.3 MB
EGAF00000595395 cram 200.1 MB
EGAF00000595396 cram 60.4 MB
EGAF00000595397 cram 158.6 MB
EGAF00000595398 cram 183.1 MB
EGAF00000595399 cram 177.1 MB
EGAF00000595400 cram 267.3 MB
EGAF00000595401 cram 223.7 MB
EGAF00000595402 cram 241.7 MB
EGAF00000595403 cram 250.2 MB
EGAF00000595404 cram 236.8 MB
EGAF00000595405 cram 235.5 MB
EGAF00000595406 cram 271.2 MB
EGAF00000595407 cram 216.6 MB
EGAF00000595408 cram 191.8 MB
EGAF00000595409 cram 179.3 MB
EGAF00000595410 cram 204.8 MB
EGAF00000595411 cram 224.0 MB
EGAF00000595412 cram 213.4 MB
EGAF00000595413 cram 244.4 MB
EGAF00000595414 cram 282.4 MB
EGAF00000595415 cram 329.5 MB
EGAF00000595416 cram 217.1 MB
EGAF00000595417 cram 273.9 MB
EGAF00000595418 cram 281.6 MB
EGAF00000595419 cram 220.3 MB
EGAF00000595420 cram 198.2 MB
EGAF00000595421 cram 232.5 MB
EGAF00000595422 cram 224.4 MB
EGAF00000595423 cram 235.4 MB
EGAF00000595424 cram 368.0 MB
EGAF00000595425 cram 342.1 MB
EGAF00000595426 cram 157.7 MB
EGAF00000595427 cram 228.2 MB
EGAF00000595428 cram 253.3 MB
EGAF00000595429 cram 338.2 MB
EGAF00000595430 cram 190.2 MB
EGAF00000595431 cram 246.5 MB
EGAF00000595432 cram 231.6 MB
EGAF00000595433 cram 220.1 MB
EGAF00000595434 cram 153.6 MB
EGAF00000595435 cram 173.2 MB
EGAF00000595436 cram 250.5 MB
EGAF00000595437 cram 241.0 MB
EGAF00000595438 cram 213.0 MB
EGAF00000595439 cram 260.5 MB
EGAF00000595440 cram 188.9 MB
EGAF00000595441 cram 223.3 MB
EGAF00000595442 cram 259.1 MB
EGAF00000595443 cram 196.6 MB
EGAF00000595444 cram 270.5 MB
EGAF00000595445 cram 202.5 MB
EGAF00000595446 cram 205.3 MB
EGAF00000595447 cram 211.2 MB
EGAF00000595448 cram 343.0 MB
EGAF00000595449 cram 211.9 MB
EGAF00000595450 cram 261.0 MB
EGAF00000595451 cram 172.4 MB
EGAF00000595452 cram 240.9 MB
EGAF00000595453 cram 267.8 MB
EGAF00000595454 cram 271.9 MB
EGAF00000595455 cram 225.1 MB
EGAF00000595456 cram 241.9 MB
EGAF00000595457 cram 252.2 MB
EGAF00000595458 cram 184.2 MB
EGAF00000595459 cram 239.4 MB
EGAF00000595460 cram 225.8 MB
EGAF00000595461 cram 334.5 MB
EGAF00000595462 cram 252.1 MB
EGAF00000595463 cram 194.0 MB
EGAF00000595464 cram 267.9 MB
EGAF00000595465 cram 280.9 MB
EGAF00000595466 cram 229.6 MB
EGAF00000595467 cram 290.5 MB
EGAF00000595468 cram 305.9 MB
EGAF00000595469 cram 171.6 MB
EGAF00000595470 cram 156.9 MB
EGAF00000595471 cram 237.3 MB
EGAF00000684353 cram 189.1 MB
EGAF00000684354 cram 11.3 MB
EGAF00000684355 cram 228.6 MB
EGAF00000684356 cram 128.5 MB
EGAF00000684357 cram 179.6 MB
EGAF00000684358 cram 165.1 MB
EGAF00000684359 cram 183.1 MB
EGAF00000684360 cram 119.1 MB
EGAF00000684361 cram 1.9 MB
EGAF00000684362 cram 58.1 MB
EGAF00000684363 cram 100.8 MB
EGAF00000684364 cram 155.2 MB
EGAF00000684365 cram 279.4 MB
EGAF00000684366 cram 142.0 MB
EGAF00000684367 cram 215.9 MB
EGAF00000684368 cram 172.7 MB
EGAF00000684369 cram 198.9 MB
EGAF00000684370 cram 154.8 MB
EGAF00000684371 cram 240.0 MB
EGAF00000684372 cram 171.4 MB
EGAF00000684373 cram 17.7 MB
EGAF00000684374 cram 95.3 MB
EGAF00000684375 cram 347.3 MB
EGAF00000684376 cram 213.4 MB
EGAF00000684377 cram 88.2 MB
EGAF00000684378 cram 127.7 MB
EGAF00000684379 cram 202.6 MB
EGAF00000684380 cram 277.9 MB
EGAF00000684381 cram 141.3 MB
EGAF00000684382 cram 213.8 MB
EGAF00000684383 cram 171.9 MB
EGAF00000684384 cram 198.2 MB
EGAF00000684385 cram 154.2 MB
EGAF00000684386 cram 238.3 MB
EGAF00000684387 cram 170.7 MB
EGAF00000684388 cram 17.8 MB
EGAF00000684389 cram 94.7 MB
EGAF00000684390 cram 345.2 MB
EGAF00000684391 cram 212.4 MB
EGAF00000684392 cram 87.9 MB
EGAF00000684393 cram 127.4 MB
EGAF00000684394 cram 201.1 MB
EGAF00000684395 cram 154.4 MB
EGAF00000684396 cram 114.9 MB
EGAF00000684397 cram 92.1 MB
EGAF00000684398 cram 106.5 MB
EGAF00000684399 cram 147.4 MB
EGAF00000684400 cram 89.7 MB
EGAF00000684401 cram 157.9 MB
EGAF00000684402 cram 149.4 MB
EGAF00000684403 cram 134.4 MB
EGAF00000684404 cram 128.0 MB
EGAF00000684405 cram 159.4 MB
EGAF00000684406 cram 128.0 MB
EGAF00000684480 cram 155.3 MB
EGAF00000684481 cram 115.6 MB
EGAF00000684482 cram 92.3 MB
EGAF00000684483 cram 106.8 MB
EGAF00000684484 cram 148.2 MB
EGAF00000684485 cram 90.0 MB
EGAF00000684486 cram 158.3 MB
EGAF00000684487 cram 150.4 MB
EGAF00000684488 cram 135.5 MB
EGAF00000684489 cram 128.8 MB
EGAF00000684490 cram 160.9 MB
EGAF00000684491 cram 128.7 MB
EGAF00000684492 cram 184.0 MB
EGAF00000684493 cram 205.9 MB
EGAF00000684494 cram 154.7 MB
EGAF00000684495 cram 145.0 MB
EGAF00000684496 cram 144.4 MB
EGAF00000684497 cram 6.0 MB
EGAF00000684498 cram 138.3 MB
EGAF00000684499 cram 225.5 MB
EGAF00000684500 cram 181.2 MB
EGAF00000684501 cram 229.0 MB
EGAF00000684502 cram 66.8 MB
EGAF00000684503 cram 190.7 MB
EGAF00000684504 cram 164.8 MB
EGAF00000684505 cram 256.0 MB
EGAF00000684506 cram 213.7 MB
EGAF00000684507 cram 182.9 MB
EGAF00000684508 cram 204.9 MB
EGAF00000684509 cram 154.5 MB
EGAF00000684510 cram 144.6 MB
EGAF00000684511 cram 143.1 MB
EGAF00000684512 cram 6.0 MB
EGAF00000684513 cram 137.6 MB
EGAF00000684514 cram 224.1 MB
EGAF00000684515 cram 180.3 MB
EGAF00000684516 cram 228.0 MB
EGAF00000684517 cram 66.8 MB
EGAF00000684518 cram 189.2 MB
EGAF00000684519 cram 163.9 MB
EGAF00000684520 cram 254.9 MB
EGAF00000684521 cram 212.5 MB
EGAF00000684522 cram 188.3 MB
EGAF00000684523 cram 169.4 MB
EGAF00000684524 cram 972.3 kB
EGAF00000684525 cram 184.3 MB
EGAF00000684526 cram 208.0 MB
EGAF00000684527 cram 149.3 MB
EGAF00000684528 cram 64.8 MB
EGAF00000684529 cram 18.2 MB
EGAF00000684530 cram 229.8 MB
EGAF00000684531 cram 236.2 MB
EGAF00000684532 cram 230.5 MB
EGAF00000684533 cram 119.9 MB
EGAF00000684688 cram 189.0 MB
EGAF00000684689 cram 170.3 MB
EGAF00000684690 cram 982.3 kB
EGAF00000684691 cram 185.1 MB
EGAF00000684692 cram 209.7 MB
EGAF00000684693 cram 150.0 MB
EGAF00000684694 cram 65.1 MB
EGAF00000684695 cram 18.2 MB
EGAF00000684696 cram 231.5 MB
EGAF00000684697 cram 237.5 MB
EGAF00000684698 cram 231.9 MB
EGAF00000684699 cram 120.9 MB
EGAF00000684700 cram 144.2 MB
EGAF00000684701 cram 60.5 MB
EGAF00000684702 cram 2.7 MB
EGAF00000684703 cram 110.4 MB
EGAF00000684704 cram 168.5 MB
EGAF00000684705 cram 269.2 MB
EGAF00000684706 cram 75.4 MB
EGAF00000684707 cram 92.6 MB
EGAF00000684708 cram 142.6 MB
EGAF00000684709 cram 232.1 MB
EGAF00000684710 cram 208.2 MB
EGAF00000684711 cram 155.3 MB
EGAF00000684712 cram 143.4 MB
EGAF00000684713 cram 100.1 MB
EGAF00000684714 cram 41.7 MB
EGAF00000684715 cram 143.9 MB
EGAF00000684716 cram 60.2 MB
EGAF00000684717 cram 2.7 MB
EGAF00000684718 cram 109.6 MB
EGAF00000684719 cram 167.6 MB
EGAF00000684720 cram 268.1 MB
EGAF00000684721 cram 75.2 MB
EGAF00000684722 cram 92.0 MB
EGAF00000684723 cram 141.5 MB
EGAF00000684724 cram 231.5 MB
EGAF00000684725 cram 207.2 MB
EGAF00000684726 cram 154.5 MB
EGAF00000684727 cram 142.6 MB
EGAF00000684728 cram 99.6 MB
EGAF00000684729 cram 41.6 MB
EGAF00000684730 cram 151.2 MB
EGAF00000684731 cram 175.1 MB
EGAF00000684732 cram 181.1 MB
EGAF00000684860 cram 269.0 MB
EGAF00000684861 cram 86.0 MB
EGAF00000684862 cram 255.9 MB
EGAF00000684863 cram 192.5 MB
EGAF00000684864 cram 220.0 MB
EGAF00000684865 cram 151.5 MB
EGAF00000684866 cram 124.4 MB
EGAF00000684867 cram 110.6 MB
EGAF00000684868 cram 6.3 MB
EGAF00000684869 cram 91.9 MB
EGAF00000684870 cram 179.4 MB
EGAF00000684871 cram 166.4 MB
EGAF00000684872 cram 151.7 MB
EGAF00000684873 cram 176.2 MB
EGAF00000684874 cram 181.7 MB
EGAF00000684875 cram 266.7 MB
EGAF00000684876 cram 85.6 MB
EGAF00000684877 cram 253.7 MB
EGAF00000684878 cram 191.6 MB
EGAF00000684879 cram 219.2 MB
EGAF00000684880 cram 151.0 MB
EGAF00000684881 cram 123.6 MB
EGAF00000684882 cram 109.7 MB
EGAF00000684883 cram 6.3 MB
EGAF00000684884 cram 91.5 MB
EGAF00000684885 cram 178.5 MB
EGAF00000684886 cram 165.7 MB
EGAF00000684887 cram 188.0 MB
EGAF00000684888 cram 11.3 MB
EGAF00000684889 cram 226.5 MB
EGAF00000684890 cram 128.0 MB
EGAF00000684891 cram 178.9 MB
EGAF00000684892 cram 164.5 MB
EGAF00000684893 cram 182.7 MB
EGAF00000684894 cram 118.7 MB
EGAF00000684895 cram 1.9 MB
EGAF00000684896 cram 58.0 MB
EGAF00000684897 cram 100.6 MB
EGAF00000684898 cram 154.0 MB
384 Files (70.8 GB)